Insertion junction: LMJ.RY0402.065138_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.065138
Chromosome:chromosome 2
Location:2411245
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g091400 CLPB2 (1 of 1) IPR001270//IPR003593//IPR003959//IPR024064//IPR027417 - ClpA/B family // AAA+ ATPase domain // ATPase, AAA-type, core // FdhE-like // P-loop containing nucleoside triphosphate hydrolase; ClpB chaperone, Hsp100 family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCCGGCGCAGCCGCTTCCCTCTCCAAC
Internal bar code:TGTCCGAAGTGTTCTTTTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:72.9537
LEAP-Seq n confirming:205
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCATCTCCAACACACAC
Suggested primer 2:GGGTAAAGCCACACACACCT