| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.065180 |
| Chromosome: | chromosome_11 |
| Location: | 1540701 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467753 | HMO2 | Heme oxygenase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTCCAGACCAAATTGCAGGTGCGGGTGC |
| Internal bar code: | ACAACCTCCTGACCGCGCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.7494 |
| LEAP-Seq n confirming: | 7960 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCGTGTGACGCTAGAA |
| Suggested primer 2: | GGCCTTCAAGAAGCAGTTTG |