| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065205 |
| Chromosome: | chromosome 16 |
| Location: | 7760825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692228 | MARS1 | Mutant Affecting Retrograde Signaling 1; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGGGATTTCCACAGTCCACAGTAGCTG |
| Internal bar code: | GGCACTACGGCCGGGGCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 99.1115 |
| LEAP-Seq n confirming: | 3235 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCTAAACTGCTGCCTG |
| Suggested primer 2: | CCAAGCTTTAGGGTGGCATA |