| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065246 |
| Chromosome: | chromosome 15 |
| Location: | 511052 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636896 | (1 of 1) PTHR11082//PTHR11082:SF29 - TRNA-DIHYDROURIDINE SYNTHASE // TRNA-DIHYDROURIDINE SYNTHASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTTAAACTATTGATGCTGCTACGTCGT |
| Internal bar code: | CCCGACAACTCGGGTCGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 98.8652 |
| LEAP-Seq n confirming: | 2091 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACTGCGGTTTAGGGGAT |
| Suggested primer 2: | TGGATGAGTGCGACAGCTAC |