| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065284 |
| Chromosome: | chromosome_10 |
| Location: | 219775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g419200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACCCAGGCACTACAGCCATCGCTACGGCC |
| Internal bar code: | CCGTAGTATCAGGAACGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 92.0437 |
| LEAP-Seq n confirming: | 1770 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGGGTCACTACCAAATG |
| Suggested primer 2: | CCCATAGGCCATAGCACACT |