| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065292 |
| Chromosome: | chromosome 3 |
| Location: | 2458843 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159400 | (1 of 2) 2.7.7.73 - Sulfur carrier protein ThiS adenylyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGTGGGCAGTCATGCCGCTCACCTCC |
| Internal bar code: | TGCTTCTGGTCCTGTTAAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.594 |
| LEAP-Seq n confirming: | 9321 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTGTCGATGTTGTCGAT |
| Suggested primer 2: | GCGCTTGTGATATTTGAGCA |