| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065355 |
| Chromosome: | chromosome 6 |
| Location: | 1731526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261750 | BES3,RBMP1 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); RuBisCO binding membrane protein 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCTAACCCCCAAACCCTTAAACCCTA |
| Internal bar code: | CGGACAATTGGGTCTGGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 99.6727 |
| LEAP-Seq n confirming: | 9439 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGAAGGTCACCGCCTGT |
| Suggested primer 2: | TTGTTTATGACTTCGCGCTG |