| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.065487 |
| Chromosome: | chromosome 9 |
| Location: | 5394779 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399960 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCGCGTCAAACGCCCGTGTCCATCCG |
| Internal bar code: | AATTAGATGACGCAACTGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.6305 |
| LEAP-Seq n confirming: | 1618 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGGCGAAAGTCTTAGC |
| Suggested primer 2: | GGGCATCATTACGCTCATCT |