| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.065501 |
| Chromosome: | chromosome 6 |
| Location: | 8175336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305302 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGGCTCCAGGTGAAATTGCCGGATGC |
| Internal bar code: | ACGGGCTAAGCAAAGCGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 97.9972 |
| LEAP-Seq n confirming: | 2104 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATAAAGGACGTGTTGGT |
| Suggested primer 2: | TGGTAACAAAACCTGTGCCA |