| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065507 |
| Chromosome: | chromosome_5 |
| Location: | 1184431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245500 | FAP175,ANK24 | Flagellar Associated Protein with ankyrin repeats | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGTGTGCTGGCGGTCGCTCGGCTCATTG |
| Internal bar code: | CAGACCTCCCCCCGCCCGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.4844 |
| LEAP-Seq n confirming: | 9841 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACCGCAAGCTTTGTCT |
| Suggested primer 2: | TCGAACCGTGTCGGTATGTA |