| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065534 |
| Chromosome: | chromosome 2 |
| Location: | 5664078 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109683 | (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGGCCTTACCAAATACTACTTGCGGTT |
| Internal bar code: | GTGCGTGTCAGGAGTGGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 93.0863 |
| LEAP-Seq n confirming: | 3743 |
| LEAP-Seq n nonconfirming: | 278 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCTCCCGTTTGACCAT |
| Suggested primer 2: | ACGCCCAACCCTAAAGAAGT |