| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.065545 |
| Chromosome: | chromosome 16 |
| Location: | 6899813 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676533 | (1 of 1) IPR000742//IPR001599 - EGF-like domain // Alpha-2-macroglobulin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTAAGGAATGGTAGACGGGGGCCGGTT |
| Internal bar code: | ACTCAACCCCCCTGGCTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.3964 |
| LEAP-Seq n confirming: | 10209 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCACCTGTCCACACAAT |
| Suggested primer 2: | TATGCCAAGAGCTGCAAATG |