Insertion junction: LMJ.RY0402.065549_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.065549
Chromosome:chromosome 7
Location:4468251
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g343333 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGGGGTTGGTGGACCAACCACGCGACAG
Internal bar code:CCCGAATTTCCGACGAGGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:859
LEAP-Seq percent confirming:91.081
LEAP-Seq n confirming:4575
LEAP-Seq n nonconfirming:448
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATCTCTTTTCTGTCCCC
Suggested primer 2:GTAGTTGTAGAGCGCCAGGG