Insertion junction: LMJ.RY0402.065554_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.065554
Chromosome:chromosome 1
Location:3698523
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g023750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGAACGCCTCTCCCGGTCGCCCCTTGC
Internal bar code:CCTGCGACTTGTAGCTGACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:96.1471
LEAP-Seq n confirming:9358
LEAP-Seq n nonconfirming:375
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCATTTGTGAGGGTGAAG
Suggested primer 2:ACACACCAAACACTCTCCCC