Insertion junction: LMJ.RY0402.065570_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.065570
Chromosome:chromosome 1
Location:4642162
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031900 (1 of 3) PTHR10566//PTHR10566:SF76 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // PROTEIN Y32H12A.7 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACACCTTTACAACACTCGCACAACTTA
Internal bar code:GATGAATGCTTCGCTTTGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:842
LEAP-Seq percent confirming:99.6531
LEAP-Seq n confirming:5745
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGTGCTTCCTGTCCCTC
Suggested primer 2:GCACATGACACCAGCAGAGT