Insertion junction: LMJ.RY0402.065600_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.065600
Chromosome:chromosome 1
Location:47738
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g000200 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain; Esterase, SGNH hydrolase-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACCCCCAACACCCACCGTGAAGTCTG
Internal bar code:CTCCCGTGAACTCGCAGCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:548
LEAP-Seq percent confirming:53.9914
LEAP-Seq n confirming:629
LEAP-Seq n nonconfirming:536
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTAGACACGGGCATCAAGG
Suggested primer 2:AACCAAACATCGAAGCAAGG