| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065609 |
| Chromosome: | chromosome 6 |
| Location: | 6650061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294050 | (1 of 2) PTHR22640:SF2 - STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGCAAGGTGTTCGTCAGGTGGTCGGC |
| Internal bar code: | AGATGGGGAATTCTGCGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 97.7667 |
| LEAP-Seq n confirming: | 394 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCCATCGTACAAGGAC |
| Suggested primer 2: | TATCAATGGCTTACTCCCGC |