| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.065618 |
| Chromosome: | chromosome 14 |
| Location: | 3214311 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629150 | CYG4 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGGTCATTTCTTTCTCGTCCGGGTGA |
| Internal bar code: | TCTGGAGAGGGGATGGGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 87.688 |
| LEAP-Seq n confirming: | 4081 |
| LEAP-Seq n nonconfirming: | 573 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGGTATGGGCGTGAGT |
| Suggested primer 2: | GATTCCCTGTCGCTCAAGTC |