| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065641 |
| Chromosome: | chromosome 3 |
| Location: | 2017915 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156100 | (1 of 1) K11984 - U4/U6.U5 tri-snRNP-associated protein 1 (SART1, HAF, SNU66) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTCAGGGATGGCCAGGCCGCACAGCA |
| Internal bar code: | CATCGAACCCGAGCCGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 99.6479 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTAGCATTGAGGAGACG |
| Suggested primer 2: | GCCTTTCATGGACAGCTACC |