| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.065645 |
| Chromosome: | chromosome 3 |
| Location: | 7834428 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202875 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCTCAACCCCGCATCCCTAACCCCGT |
| Internal bar code: | GTGGGAGTGTGGCCCGCTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 72.4677 |
| LEAP-Seq n confirming: | 1345 |
| LEAP-Seq n nonconfirming: | 511 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGGATGGTGATTTGAC |
| Suggested primer 2: | AGTATGGGACACGTCGAAGG |