Insertion junction: LMJ.RY0402.065665_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.065665
Chromosome:chromosome 2
Location:5079512
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g104800 HTV1 (1 of 35) IPR000164//IPR007125//IPR009072 - Histone H3/CENP-A // Histone H2A/H2B/H3 // Histone-fold; Histone H3 variant 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGTTTGTCTGAAGACGGCACAGAGCGAG
Internal bar code:TGTTCGACCCCTGGCATCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:854
LEAP-Seq percent confirming:93.029
LEAP-Seq n confirming:2629
LEAP-Seq n nonconfirming:197
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACACGGTTGACAGTTGG
Suggested primer 2:TTGGGAAGTTGCGTGTGTAA