| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065685 |
| Chromosome: | chromosome 3 |
| Location: | 2264190 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157900 | (1 of 1) PTHR18937//PTHR18937:SF162 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGGTGCTTGTGCAAACCCAGCGCACTC |
| Internal bar code: | GGGCGCGGACGAAGGTAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.2949 |
| LEAP-Seq n confirming: | 3098 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGAGGTGAACGCACAG |
| Suggested primer 2: | AGCTCCGACACCTAACGAAA |