Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.065707 |
Chromosome: | chromosome 2 |
Location: | 1801250 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g086887 | NUP98-96,NUP189 | Nucleoporin 98-96; (1 of 1) K14297 - nuclear pore complex protein Nup98-Nup96 (NUP98, ADAR2, NUP116) | intron|outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAAACTAGTGCGGCAGCCTCTGTTTCC |
Internal bar code: | ATTAGGAGTGTAGATGGAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 847 |
LEAP-Seq percent confirming: | 99.4737 |
LEAP-Seq n confirming: | 756 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGTTCGAGGTGGAGCACT |
Suggested primer 2: | AACCGCACTTTCAATTCCAC |