| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.065711 |
| Chromosome: | chromosome 12 |
| Location: | 1604757 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486400 | (1 of 1) PTHR22957//PTHR22957:SF26 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // PROTEIN TBC-3, ISOFORM B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTGCGAGTTCTGGAACGATAAACACA |
| Internal bar code: | AGAAGTAGTGGGGGGAGAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.5669 |
| LEAP-Seq n confirming: | 5288 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTGTTCGCGAATACCTG |
| Suggested primer 2: | TGGTTCTGGGTCTTGGTTTC |