| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065758 |
| Chromosome: | chromosome 3 |
| Location: | 8453754 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199871 | (1 of 1) PTHR23050:SF186 - CENTRIN-4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCACAGGACTCCAGGTGCCCTCCTCAT |
| Internal bar code: | ATTCGCAGCCCCCCTTCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.5586 |
| LEAP-Seq n confirming: | 4511 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAAACGCAAGCCCTATG |
| Suggested primer 2: | TAGTGCGTCCACTTACACGG |