Insertion junction: LMJ.RY0402.065759_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.065759
Chromosome:chromosome 9
Location:4756463
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g397216 (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTGTGTTGATGAAACAACAGAAGC
Internal bar code:GTGCGGTACGCACAGGCCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:99
LEAP-Seq percent confirming:38.855
LEAP-Seq n confirming:1337
LEAP-Seq n nonconfirming:2104
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCCTCACAACCCGTCTAT
Suggested primer 2:ACACTCGCACACTTGCTCAC