| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065797 |
| Chromosome: | chromosome_2 |
| Location: | 747799 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g078550 | FAP210 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCGCGCGGCGGCCTTGCCAAACCCCCGTC |
| Internal bar code: | TCTGGGACTCAGAGGGCGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 97.844 |
| LEAP-Seq n confirming: | 2133 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAAACGCGTTCCCTTAT |
| Suggested primer 2: | GTCAACTTCGAGAGGCAAGG |