| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065803 |
| Chromosome: | chromosome 16 |
| Location: | 4778035 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686500 | (1 of 1) PF03767 - HAD superfamily, subfamily IIIB (Acid phosphatase) (Acid_phosphat_B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGTCATGCCATGCCCCACCCCCAGAT |
| Internal bar code: | GACGTTGCATCATGTTAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 91.0546 |
| LEAP-Seq n confirming: | 967 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTGCGGGTATGGAGAA |
| Suggested primer 2: | CCTACTTGGCTTGAGGCTTG |