| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065886 |
| Chromosome: | chromosome 9 |
| Location: | 1739774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396650 | PAT2 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAATTGAGGGCACGGTGTCCTAGTCTAG |
| Internal bar code: | ACATTAGATGTAGCTGTAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 96.0203 |
| LEAP-Seq n confirming: | 5115 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCCGAGAGGAAGAGGGT |
| Suggested primer 2: | TGCAGGTGCTAACGAAGATG |