| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065886 |
| Chromosome: | chromosome 11 |
| Location: | 3717410 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482800 | STK5,STPK5 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Serine/threonine protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGCGTAGGCCATGACGCGGGACCGCGA |
| Internal bar code: | CCGGCTGGCTTTGCGGGTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTGTGCCACGTCAATG |
| Suggested primer 2: | CCAGGTCTGACCAACTCCAT |