Insertion junction: LMJ.RY0402.065911_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.065911
Chromosome:chromosome 6
Location:6447709
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g292700 SNRK2G snRK1 family in Chlamydomonas, subgroup 2; (1 of 6) K14498 - serine/threonine-protein kinase SRK2 (SNRK2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAGTGATGCCAGCAATGCCAAGATAGC
Internal bar code:CCTTACGGGTGTATGTCCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:606
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGCATAACCTATCGAGC
Suggested primer 2:ACGGTCTTCTGGTTGTGGAG