| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.065914 |
| Chromosome: | chromosome 7 |
| Location: | 5728203 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352800 | (1 of 1) PTHR35460:SF1 - TRANSLATION ELONGATION FACTOR EF-1 ALPHA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCACCCCGCACACAGCTCCCAGGCA |
| Internal bar code: | AGCCCAGTATGACCTTTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTACGTGTGTCGC |
| Suggested primer 2: | AAGACTGTGCGAGTGTGTGC |