| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065934 |
| Chromosome: | chromosome 3 |
| Location: | 5262428 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183300 | (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTCTACCAGACGGGTGTTGCACTCGC |
| Internal bar code: | AGCATTGCAGCCCATCGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 95.3642 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAACTGTGATGCTGAAGA |
| Suggested primer 2: | ATCACGTCCGGGTGATATGT |