Insertion junction: LMJ.RY0402.066000_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.066000
Chromosome:chromosome 11
Location:1167764
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCGGCGTGCCGGGTGCGTTGCCGACT
Internal bar code:CGCAGCGCACTATCTGAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:735
LEAP-Seq percent confirming:99.7373
LEAP-Seq n confirming:7972
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAACTGGGTCGTACACTT
Suggested primer 2:ATCTTCGGAGGGTACGTGTG