| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066015 |
| Chromosome: | chromosome 3 |
| Location: | 6585268 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196150 | uL5m,MRPL5 | (1 of 3) IPR022803 - Ribosomal protein L5 domain; Mitochondrial ribosomal protein L5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAACCTGAACCCCGCACCTATCAAGCA |
| Internal bar code: | CGACTGCCGTCTCCGACGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.8282 |
| LEAP-Seq n confirming: | 581 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCGCACTTCACAGAGAT |
| Suggested primer 2: | GTTTCGTTCGCAATGGATTT |