| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066044 |
| Chromosome: | chromosome_10 |
| Location: | 4256002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g450450 | FAP18 | Flagellar Associated Protein, serine endopeptidase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGACCGCAGCGTGACCGACAGCGACCGCA |
| Internal bar code: | CTGCCCTCAGGGGTACCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 96.6979 |
| LEAP-Seq n confirming: | 2167 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGGCACCTACAAGCTG |
| Suggested primer 2: | GTCGTGCTGCCCTCTAAAAG |