Insertion junction: LMJ.RY0402.066045_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066045
Chromosome:chromosome 16
Location:3812384
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g689600 MIA2,FAP73 (1 of 2) PF13863 - Domain of unknown function (DUF4200) (DUF4200); MIA2 subunit of the modifier of inner arms (MIA) complex CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCATCGTTTGCTTTCTTCTCTGCTCGTG
Internal bar code:ATCTCCGACGTCCCTCGAACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:99.7625
LEAP-Seq n confirming:840
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCTGGTTGTTGAGGTTT
Suggested primer 2:AACACACAGAAGCAAACCCC