Insertion junction: LMJ.RY0402.066051_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066051
Chromosome:chromosome 13
Location:3743526
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTGGCCGCAATCGCCTCATCGTCCACC
Internal bar code:ACCGATGTATTTGCGGGGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:779
LEAP-Seq percent confirming:99.5647
LEAP-Seq n confirming:4346
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGCTGTCGAAAACTCACC
Suggested primer 2:GTTTGGATCCAGCCACAACT