| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066054 |
| Chromosome: | chromosome 4 |
| Location: | 2487418 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGACAGCCGCGACAATTTGACAGATGC |
| Internal bar code: | ACCCCGATCTGGGTCTGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 19 |
| LEAP-Seq percent confirming: | 68.2274 |
| LEAP-Seq n confirming: | 204 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGAGTCCTTTTCAGTTTG |
| Suggested primer 2: | CAGCTTTAACTTGGGCTTGC |