| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066100 |
| Chromosome: | chromosome 1 |
| Location: | 93984 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000650 | AMX2 | (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGCTCACCACCAGCCGCCTGCTGCGC |
| Internal bar code: | ATGATGTAGCAGCAAGCCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 98.7754 |
| LEAP-Seq n confirming: | 11050 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCACCTACATGGACGCA |
| Suggested primer 2: | CACTTCCCTGTCCTCCTCAA |