| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066119 |
| Chromosome: | chromosome 13 |
| Location: | 4092971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAACCCATCCCGGGGGGTTGGGGAGCA |
| Internal bar code: | CGCTCGGAAACCGAGCTGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 96.2063 |
| LEAP-Seq n confirming: | 2891 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACAGGACCAAGAGGGAA |
| Suggested primer 2: | AGTGAGTGGGTCAAGTTGGG |