| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066154 |
| Chromosome: | chromosome 12 |
| Location: | 5225830 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528100 | CPZ | Zinc carboxypeptidase; (1 of 1) PTHR12756:SF12 - CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTGGACGTAGCGTTGACACCCACGGTT |
| Internal bar code: | GACCCCGGTCCTGCTATATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 78.2027 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 228 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGAACGTAAGAGGCGG |
| Suggested primer 2: | CGAGGTGTTGTAATTGGCCT |