| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066166 |
| Chromosome: | chromosome 7 |
| Location: | 4618630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344200 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGGCTGTCGAGGTGTGTGTGCGTGTG |
| Internal bar code: | GTGGACCGGATCTCCACAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 96.8214 |
| LEAP-Seq n confirming: | 8072 |
| LEAP-Seq n nonconfirming: | 265 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGGCTGTAGTAGTTGC |
| Suggested primer 2: | CCAGTCTTCTCGTGCTTTCC |