| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066191 |
| Chromosome: | chromosome 7 |
| Location: | 5340470 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349950 | (1 of 1) K03131 - transcription initiation factor TFIID subunit 6 (TAF6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCGCCCCCCGCATGCGCACGCAGGTGT |
| Internal bar code: | CCGACGGAGTGCTCGTCCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 151 |
| LEAP-Seq percent confirming: | 43.0397 |
| LEAP-Seq n confirming: | 1280 |
| LEAP-Seq n nonconfirming: | 1694 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGTTCCTCACCCACAT |
| Suggested primer 2: | GAACAAGAGGGTGGTCTGGA |