| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066192 |
| Chromosome: | chromosome 2 |
| Location: | 4885977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103400 | OPR118 | OctotricoPeptide Repeat protein 118 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACATTAACTTGTCGTGTGCACGGCTC |
| Internal bar code: | TATTACCAGGGGGGACGGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 95.9288 |
| LEAP-Seq n confirming: | 377 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACAGGTTGCGGTAGGTT |
| Suggested primer 2: | GAAGGTTGCCTGACCTTTTG |