| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066197 |
| Chromosome: | chromosome 4 |
| Location: | 2688662 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTGCGGGCATTCCTTCTTATCAACGG |
| Internal bar code: | CGAGGGGTCGAGGGGCGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 41.4309 |
| LEAP-Seq n confirming: | 2050 |
| LEAP-Seq n nonconfirming: | 2898 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTTGCTTCTCTCCATGT |
| Suggested primer 2: | TTGCAGTGGCATCAAAAGAG |