| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066228 |
| Chromosome: | chromosome 1 |
| Location: | 1045000 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006050 | LF3 | Long Flagella protein 3; (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGGCAGCGCCGCAGCTGCCTGAGCGCC |
| Internal bar code: | AACCTACAGGGAAGCGCAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTCTAGCAGGGACAGC |
| Suggested primer 2: | AACTCAACTCCCATGCAGGT |