Insertion junction: LMJ.RY0402.066238_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.066238
Chromosome:chromosome 17
Location:4339486
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g731100 CPL14 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); Conserved in the Plant Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGAACACTGGCCGCTATTATTCTTAGCA
Internal bar code:ATGTCCTGGTCGTTGAGAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:99.4168
LEAP-Seq n confirming:2216
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGACTCAATACCTGGGGC
Suggested primer 2:CCATACATCCCCACCTTCAC