Insertion junction: LMJ.RY0402.066246_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.066246
Chromosome:chromosome 6
Location:5956576
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g288900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATAAAAGTACACCTCTCTATTGAGTAAA
Internal bar code:GCAAGTCACTTCCGGGGGTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:112
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGGTGAGGCATGCAGTAG
Suggested primer 2:TGATGTACTTTGCTCGCAGG