| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066293 |
| Chromosome: | chromosome 17 |
| Location: | 1092885 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703932 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCAATCGAGAGGATTCTTGTGACAGCAA |
| Internal bar code: | ACGGCATTTCTGCATGCGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 93.3162 |
| LEAP-Seq n confirming: | 6534 |
| LEAP-Seq n nonconfirming: | 468 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTTCCAAATGTGCCTT |
| Suggested primer 2: | GATGGCCGTGTAGTATGGCT |